Supercritical Fluid Chromatography (SFC)

Nicotine and alkaloids in tobacco separated by SFC for analysis.

HPLC: 2 pumps provide mixed mobile phase,

packed column in oven,

pressure and flow-rates controlled.

GC: syringe pump & restrictor,

flame-ionization detector.

SFC: Higher flow rates

Liquid-like solubility

SFC: Lower Temperatures

• Successful for analytes that won’t vaporize, nor have functional groups for detection by HPLC detectors.

• High resolution & sensitivity.

• Investigation of SFC for enantiomeric separation using chiral stationary phase.

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