•Up to tens of thousands of DNA probes are covalently attached to
glass by epoxysilane
coupling agent
•mRNA
isolated from cell cultures is converted into fluorescently-labeled DNA
•Homologous,
labeled DNA targets bind DNA probes
•Non-homologous targets are prevented from binding by 62°C
hybridization temperature
and subsequent washes
•Fluorescent
excitation is used to assess binding events