13
Microarray
Measurement of Differential Gene Expression
Control Cells
Cells + Treatment
RNA isolation
Cy-3 label
Cy-5 label
Reverse transcription
Mix cDNAs and apply to array. Hybridize under coverslip.
Cy-3 labeled cDNA
Cy-5 labeled cDNA
Spots
with more Cy3 are genes down-regulated by
treatment
Spots
with more
Cy5 are
genes up-regulated by treatment
Mixed spots are genes unaffected by
treatment