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Conventional Microarray Technology
Glass Substrate
DNA “probe”
DNA “target”
•Up to tens of thousands of DNA probes are covalently attached to glass by epoxysilane coupling agent
•mRNA isolated from cell cultures is converted into fluorescently-labeled DNA
•Homologous, labeled DNA targets bind DNA probes
•Non-homologous targets are prevented from binding by 62°C hybridization temperature and subsequent washes
•Fluorescent excitation is used to assess binding events
Fluorescent emission
A.
B.
Glass Substrate