Microarray Measurement of Differential Gene Expression
Control
Cells
Cells +
Treatment
RNA isolation
Cy-3 label
Cy-5 label
Reverse transcription
Mix cDNAs and apply to array. Hybridize under coverslip.
Cy-3 labeled cDNA
Cy-5 labeled cDNA
Spots with more Cy3 are genes down-regulated by
treatment
Spots with more Cy5 are
genes up-regulated by treatment
Mixed spots are genes unaffected by
treatment